Singapore Institute of Technology
Browse
Characterization of Transcriptional Activities_Accepted.pdf (1.17 MB)

Characterization of Transcriptional Activities

Download (1.17 MB)
chapter
posted on 2022-09-26, 08:21 authored by Adison WongAdison Wong, Maurice H. T. Ling

Transcription is the first stage of gene expression, leading to the eventual determination of protein abundance and affecting metabolism. Hence, there is a need to measure and characterize transcriptional activities accurately. This article discusses the experimental techniques to characterize transcriptional activities from a single-gene approach (quantitative PCR) to high-throughput methods (microarray technology and next generation sequencing). Computational approaches to predict relative transcript abundance from sequence features and gene co-expression network will be described. As a proxy to protein/enzyme abundance, transcriptional activities are critical in developing simulatable biochemical models, which can then be used to test biological hypotheses prior to laboratory experimentation.

History

Journal/Conference/Book title

Encyclopedia of Bioinformatics and Computational Biology

Publication date

2019

Version

  • Post-print

Corresponding author

Maurice Ling; Adison Wong