Singapore Institute of Technology
Metabolic Engineering of Microbes for Monoterpenoids Production_Post-print.pdf (1.71 MB)

Metabolic engineering of microbes for monoterpenoid production

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journal contribution
posted on 2022-09-25, 04:32 authored by Kun Zhu, Jing Kong, Baixiang Zhao, Lanxin Rong, Shiqi Liu, Zhihui Lu, Cuiying Zhang, Dongguang Xiao, Krithi Pushpanathan, Jee Loon Foo, Adison WongAdison Wong, Aiqun Yu
Monoterpenoids are an important class of natural products that are derived from the condensation of two five‑carbon isoprene subunits. They are widely used for flavouring, fragrances, colourants, cosmetics, fuels, chemicals, and pharmaceuticals in various industries. They can also serve as precursors for the production of many industrially important products. Currently, monoterpenoids are produced predominantly through extraction from plant sources. However, the small quantity of monoterpenoids in nature renders this method of isolation non-economically viable. Similarly impractical is the chemical synthesis of these compounds as they suffer from high energy consumption and pollutant discharge. Microbial biosynthesis, however, exists as a potential solution to these hindrances, but the transformation of cells into efficient factories remains a major impediment. Here, we critically review the recent advances in engineering microbes for monoterpenoid production, with an emphasis on categorized strategies, and discuss the challenges and perspectives to offer guidance for future engineering.


Ministry of Education, Singapore (R-MOE-A401-C001, R-MOE-E103-E001)


Journal/Conference/Book title

Biotechnology Advances

Publication date



  • Post-print

Corresponding author

Adison Wong; Aiqun Yu

Project ID

  • 6525 (R-MOE-A401-C001) Developing a Scale-Up Platform for Adoptive Cell Therapy
  • 1 (R-IND-E103-0025; R-MOE-E103-E001) Cell separation using microfluidics technology

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