Singapore Institute of Technology
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Piloting a scale-up platform for high-quality human T-cells production

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posted on 2024-08-14, 10:04 authored by Viknesvaran Selvarajan, Denise Bei Lin Teo, Chaw-Chiea Chang, Yuen Ling Ng, Nge Cheong, Jaichandran Sivalingam, Soo Hean Gary Khoo, Adison WongAdison Wong, Liat Wen Bernard LooLiat Wen Bernard Loo

Cell and gene therapies are an innovative solution to various severe diseases and unfulfilled needs. Adoptive cell therapy (ACT), a form of cellular immunotherapies, has been favored in recent years due to the approval of chimeric antigen receptor CAR-T products. Market research indicates that the industry’s value is predicted to reach USD 24.4 billion by 2030, with a compound annual growth rate (CAGR) of 21.5%. More importantly, ACT is recognized as the hope and future of effective, personalized cancer treatment for healthcare practitioners and patients worldwide. The significant global momentum of this therapeutic approach underscores the urgent need to establish it as a practical and standardized method. It is essential to understand how cell culture conditions affect the expansion and differentiation of T-cells. However, there are ongoing challenges in ensuring the robustness and reproducibility of the manufacturing process. The current study evaluated various adoptive T-cell culture platforms to achieve large-scale production of several billion cells and high-quality cellular output with minimal cell death. It examined factors such as bioreactor parameters, media, supplements and stimulation. This research addresses the fundamental challenges of scalability and reproducibility in manufacturing, which are essential for making adoptive T-cell therapy an accessible and powerful new class of cancer therapeutics.

Funding

Ministry of Education Singapore

History

Journal/Conference/Book title

Frontiers in Cell and Developmental Biology

Publication date

2024-07-12

Version

  • Published

Corresponding author

Bernard Liat Wen Loo

Project ID

  • 6525 (R-MOE-A401-C001) Developing a Scale-Up Platform for Adoptive Cell Therapy

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